Life was so much easier when Twitter wasn't worried about making money. Some spectacular venture capital deals propelled the company's value to over $1 billion, and user trends shot bragging rights up proportionately. Its ascendancy resulted in large part from the efforts of the Twitter ecosystem where companies developed 70,000 applications that have made life easier or more measurable for Twitter users. It's easy to lose track of how much of our Twitter interaction lies on third-party apps – from photo sharing to smartphone applications to URL shortening.

As long as Twitter's functionality ambitions remained modest, the ecosystem knew it would thrive. As we head toward the Chirp conference for Twitter application developers concerns over the future of the ecosystem abound. Here are five reasons why the companies around Twitter may start to worry about the microblogging juggernaut.

Twitter-watchers have been expecting the company to make some move toward corporate microblogging capabilities for months now. This is one of the two directions that the social media platform has on its agenda for generating revenue, and it is likely the one with the greater potential. The other, serving ads on Twitter.com, is currently constrained by the fact that nearly 70% of Twitter use occurs away from the company's website.

While all talk of corporate accounts is still shrouded in speculation, the obvious plan would be for corporate users to have more robust analytics and other marketing tools for which a premium would be paid. Among the tools being rolled out ostensibly in advance of this business model is a multi-user account model, in which several "Contributors," as Twitter is calling them, can tweet under the same umbrella.

It's tough to take on Google (NASDAQ: GOOG). The search engine behemoth owns 65% of the U.S. search market and has a commanding brand presence. Yet, the software maker up the coast isn't known to give up easily. Microsoft (NASDAQ: MSFT) has cut a deal with microblogging site Twitter that should give it an edge in the battle to harness data and make it easier to find. A new deal will feed all those tweets into Bing, the Microsoft search engine.

Twitter is giving Microsoft full access to its data, in a deal announced Wednesday. Bing will provide search functionality for Twitter that you won't find in Google, which seems to have been outbid for the rights to the "tweet-stream." Under the deal, Bing will be able to index and display the tweets almost immediately as they are posted.